熱門關(guān)鍵詞: CO2搖床 凝膠成像系統(tǒng) 細(xì)胞培養(yǎng)耗材 乳酸分析儀 發(fā)酵罐 Rainin移液槍 振蕩培養(yǎng)箱 生物反應(yīng)器
細(xì)胞名稱: 人卵巢癌細(xì)胞(STR鑒定正確)
細(xì)胞簡稱:A2780細(xì)胞
細(xì)胞別名: A-2780; 2780; A2780S
貨號(hào):TCH-C112規(guī)格: 1X10^6 T25培養(yǎng)瓶
A2780細(xì)胞價(jià)格:1000元 (干冰運(yùn)輸需另加200元干冰費(fèi))
A2780細(xì)胞培養(yǎng)基:TCH-G112
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A2780細(xì)胞
細(xì)胞名稱: 人卵巢癌細(xì)胞(STR鑒定正確)
細(xì)胞簡稱:A2780細(xì)胞
細(xì)胞別名: A-2780; 2780; A2780S
貨號(hào):TCH-C112
規(guī)格: 1X10^6 T25培養(yǎng)瓶
價(jià)格:1000元 (干冰運(yùn)輸需另加200元干冰費(fèi))
種屬來源:人
組織來源:卵巢
特征:卵巢癌
細(xì)胞形態(tài):上皮細(xì)胞樣
生長特性:貼壁生長
培養(yǎng)體系:RPMI-1640+10%FBS+1%P/S
配套培養(yǎng)基貨號(hào):TCH-G112
傳代比例:1:3-1:6,每2-3天換液一次
傳代周期:48-72 h
培養(yǎng)條件:氣相:95%空氣+5%CO2,溫度:37℃
凍存條件:60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO,液氮儲(chǔ)存
質(zhì)量檢測:細(xì)菌、真菌、支原體檢測均為陰性
參考文獻(xiàn):
1. Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
2. Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R., Easwaran H., Baylin S.B., Slamon D., Velculescu V.E., Scharpf R.B.
Integrated genomic, epigenomic, and expression analyses of ovarian cancer cell lines.
Cell Rep. 25:2617-2633(2018)
3. Zhao R., Qin W.-J., Qin R.-H., Han J., Li C., Wang Y.-S., Xu C.-J.
Lectin array and glycogene expression analyses of ovarian cancer cell line A2780 and its cisplatin-resistant derivate cell line A2780-cp.
Clin. Proteomics 14:20-20(2017)
4. Medrano M., Communal L., Brown K.R., Iwanicki M., Normand J., Paterson J., Sircoulomb F., Krzyzanowski P., Novak M., Doodnauth S.A., Saiz F.S., Cullis J., Al-Awar R., Neel B.G., McPherson J., Drapkin R., Ailles L., Mes-Massons A.-M., Rottapel R.
Interrogation of functional cell-surface markers identifies CD151 dependency in high-grade serous ovarian cancer.
Cell Rep. 18:2343-2358(2017)
5. Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
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