人結(jié)腸癌細(xì)胞HCT116

人結(jié)腸癌細(xì)胞HCT116 是1979年BrattainM等分離建立的3株惡性細(xì)胞之一；產(chǎn)生CEA、角蛋白。在半固體瓊脂糖培養(yǎng)基中形成克隆。HCT116在無(wú)胸腺的裸鼠有致瘤性，形成上皮樣的腫瘤。

細(xì)胞名稱：人結(jié)腸癌細(xì)胞

細(xì)胞簡(jiǎn)稱：HCT116細(xì)胞

細(xì)胞別稱：HCT-116; HCT.116; HCT_116; HCT116; CoCL2

產(chǎn)品貨號(hào)：TCH-C185

種屬來(lái)源：人

組織來(lái)源：結(jié)腸

特征：結(jié)腸癌

細(xì)胞形態(tài)：上皮細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系： McCoy's 5A+10%FBS+1%P/S

配套培養(yǎng)基貨號(hào)：TCH-G185

傳代比例：1:3-1:8，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

實(shí)驗(yàn)耗材：細(xì)胞培養(yǎng)瓶，錐形搖瓶,細(xì)胞培養(yǎng)皿

實(shí)驗(yàn)室儀器：CO2培養(yǎng)箱

人結(jié)腸癌細(xì)胞HCT116 使用說(shuō)明書

參考文獻(xiàn)：

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